Protein misfolding and cellular stress in disease and aging : concepts and protocols  (hbk. ~ (eISBN)

edited by Peter Bross and Niels Gregersen

How and why certain proteins misfold and how this misfolding is linked to many disease processes has become a well-documented topic of study. Protein Misfolding and Cellular Stress in Disease and Aging: Concepts and Protocols moves beyond the basics to emphasize the molecular effects of protein misfolding at a cellular level, to delineate the impacts and cellular reactions that play a role in pathogenetic mechanisms, and to pinpoint possible manipulations and treatment strategies that can counteract, modify, or delay the consequences of misfolding. The volume begins with several concepts and approaches developed in the recent past including a connection to the research field of aging, where protein misfolding diseases have been equated to premature aging processes, and the book's coverage continues with detailed descriptions of protocols for relevant experimental approaches. Written in the highly successful Methods in Molecular Biologya series format, protocols chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Misfolding and Cellular Stress in Disease and Aging: Concepts and Protocols aims to aid researchers in the field, as well as medical professionals and molecular biologists, in shaping and performing research related to this intriguing and vital subject.

「Nielsen BookData」より


  • Part I: Concepts 1. Protein Misfolding and Cellular Stress: An Overview Niels Gregersen and Peter Bross 2. Protein Aggregation Diseases: Toxicity of Soluble Prefibrillar Aggregates and Their Clinical Significance Massimo Stefani 3. Consequences of Stress in the Secretory Pathway: The ER Stress Response and Its Role in the Metabolic Syndrome Martin Schroder and Louise Sutcliffe 4. What Role Does Mitochondrial Stress Play in Neurodegenerative Diseases? Alicia Mae Pickrell and Carlos Torres Moraes 5. Autophagy in Disease Dalibor Mijaljica, Mark Prescott, and Rodney J. Devenish 6. Mitophagy and Mitoptosis in Disease Processes Dalibor Mijaljica, Mark Prescott, and Rodney J. Devenish 7. Cellular Stress and Protein Misfolding During Aging Rajiv Vaid Basaiawmoit and Suresh I.S. Rattan 8. Measuring Consequences of Protein Misfolding and Cellular Stress Using OMICS Techniques Peter Bross, Johan Palmfeldt, Jakob Hansen, Soren Vang, and Niels Gregersen Part II: Protocols 9. Production of Cells with Targeted Integration of Gene Variants of Human ABC Transporter for Stable and Regulated Expression Using the Flp Recombinase System Kanako Wakabayashi-Nakao, Ai Tamura, Shoko Koshiba, Yu Toyoda, Hiroshi Nakagawa, and Toshihisa Ishikawa 10. Stress Response Profiles in Human Fibroblasts Exposed to Heat Shock or Oxidative Stress Christina Bak Pedersen and Niels Gregersen 11. Examining Ubiquitinated Protein Aggregates in Tissue Sections Natalia A. Kaniuk and John H. Brumell 12. Determination of Proteasomal Activities Cristovao F. Lima and Suresh I.S. Rattan 13. Measurement of Autophagy in Cells and Tissues Isei Tanida and Satoshi Waguri 14. Transcription Factor Sequestration by Polyglutamine Proteins Tomoyuki Yamanaka and Nobuyuki Nukina 15. Biological Membranes as Protein Aggregation Matrices and Targets of Amyloid Toxicity Monica Bucciantini and Cristina Cecchi 16. Measurement of Mitochondrial ROS Production Anatoly A. Starkov 17. In vivo Detectionof Oxidized Proteins: A Practical Approach to Tissue-Derived Mitochondria Francesca Maltecca and Giorgio Casari 18. Measurement of Oxidized/Reduced Glutathione Ratio Joshua B. Owen and D. Allan Butterfield 19. Determination of Altered Mitochondria Ultrastructure by Electron Microscopy Shoichi Sasaki 20. Assessing Bad Sub-Cellular Localization under Conditions Associated with Prevention or Promotion of Mitochondrial Permeability Transition-Dependent Toxicity Liana Cerioni and Orazio Cantoni 21. A Cellular Viability Assay to Monitor Drug Toxicity Jakob Hansen and Peter Bross 22. Rescue of Misfolded Proteins and Stabilization by Small Molecules Raymond C. Stevens, Javier Sancho, and Aurora Martine

「Nielsen BookData」より


書名 Protein misfolding and cellular stress in disease and aging : concepts and protocols
著作者等 Bross, Peter
Gregersen, Niels
シリーズ名 Methods in molecular biology
Springer protocols
巻冊次 (hbk.
出版元 Humana Press
刊行年月 c2010
ページ数 xi, 330 p.
大きさ 27 cm
ISBN 1607617560
NCID BB11815914
※クリックでCiNii Booksを表示
言語 英語
出版国 アメリカ合衆国


著作名 著作者名
A cellular viability assay to monitor drug toxicity Jakob Hansen, Bross, Peter
Assessing bad sub-cellular localization under conditions associated with prevention or promotion of mitochondrial permeability transition-dependent toxicity Liana Cerioni, Orazio Cantoni
Autophagy in disease Dalibor Mijaljica, Mark Prescott, Rodney J. Devenish
Biological membranes as protein aggregation matrices and targets of amyloid toxicity Cristina Cecchi, Monica Bucciantini
Cellular stress and protein misfolding during aging Rajiv Vaid Basaiawmoit, Suresh I.S. Rattan
Consequences of stress in the secretary pathway : the ER stress response and its role in the metabolic syndrome Louise Sutcliffe, Martin Schröder
Determination of altered mitochondria ultrastructure by electron microscopy Shoichi Sasaki
Determination of proteasomal activities Cristovao F. Lima, Suresh I.S. Rattan
Examining ubiquitinated protein aggregates in tissue sections John H. Brumell, Natalia A. Kaniuk
In vivo detection of oxidized proteins : a practical approach to tissue-derived mitochondria Francesca Maltecca, Giorgio Casari
Measurement of autophagy in cells and tissues Isei Tanida, Satoshi Waguri
Measurement of mitochondrial ROS production Anatoly A. Starkov
Measurement of oxidized/reduced glutathione ratio D. Allan Butterfield, Joshua B. Owen
Measuring consequences of protein misfolding and cellular stress using OMICS techniques Bross, Peter
Mitophagy and mitoptosis in disease processes Dalibor Mijaljica, Mark Prescott, Rodney J. Devenish
Production of cells with targeted integration of gene variants of human ABC transporter for stable and regulated expression using the Flp recombinase system Kanako Wakabayashi-Nakao
Protein aggregation diseases : toxicity of soluble prefibrillar aggregates and their clinical significance Massimo Stefani
Protein misfolding and celluar stress : an overview Bross, Peter, Gregersen, Niels
Rescue of misfolded proteins and stabilization by small molecules Aurora Martinez, Javier Sancho, Raymond C. Stevens
Stress response profile in human fribroblasts exposed to heat shock or oxidative stress Christina Bak Pedersen, Gregersen, Niels
Transcription factor sequestration by polyglutamine proteins Nobuyuki Nukina, Tomoyuki Yamanaka
What role does mitochondrial stress play in neurodegenerative diseases? Alicia Mae Pickrell, Carlos Torres Moraes