Recombinant DNA methods are powerful, revolutionary techniques for at least two reasons. First, they allow the isolation of single genes in large amounts from a pool of thousands or millions of genes. Second, the isolated genes or their regulatory regions can be modified at will and re-introduced into cells for expression at the RNA or protein levels. These attributes help to solve complex biological problems and to produce new and better products in the areas of health, agriculture and industry. Both volume 154 and 155 include the descriptions of several useful new restriction enzymes and rapid methods for DNA sequence analysis, along with a number of other useful methods.
Methods for Cloning cDNA. H. Okayama, M. Kawaichi, M. Brownstein, F. Lee, T. Yokota, and K. Arai, High-Efficiency Cloning of Full-Length cDNA
Construction and Screening of cDNA Expression Libraries for Mammalian Cells. G. Heidecker and J. Messing, A Method for Cloning Full-Length cDNA in Plasmid Vectors. D.C. Alexander, An Efficient Vector*b1Primer cDNA Cloning System. C. Coleclough, Use of Primer*b1Restriction End Adapters in cDNA Cloning. Identification of Cloned Genes and Mapping of Genes. A.A. Reyes and R.B. Wallace, Mapping of RNA Using S1 Nuclease and Synthetic Oligonucleotides. C.G. Miyada and R.B. Wallace, Oligonucleotide Hybridization Techniques. M. Snyder, D. Sweetser, R.A. Young, and R.W. Davis, *glgt 11: Gene Isolation with Antibody Probes and Other Applications. M.R. Gray, G.P. Mazzara, P. Reddy, M. Rosbash, Searching for Clones with Open Reading Frames. G.M. Weinstock, Use of Open Reading Frame Expression Vectors. J.D. Boeke, J. Trueheart, G. Natsoulis, and G.R. Fink, 5-Flouroorotic Acid as a Selective Agent in Yeast Molecular Genetics. F.J. de Bruijn, Transposon Tn5 Mutagenesis to Map Genes. J.D. Noti, M.N. Jagadish, and A.A. Szalay, Site-Directed Tn5 and Transplacement Mutagenesis: Methods to Identify Symbiotic Nitrogen Fixation Genes in Slow-Growing Rhizobium. Chemical Synthesis and Analysis of Oligodeoxynucleotides. R. Frank, A. Meyerhans, K. Schwellnus, and H. Blvcker, Simultaneous Synthesis and Biological Applications of DNA Fragments: An Efficient and Complete Methodology. H.W. DjurhuusYMatthes, A. Staub, and P. Chambon, The Segmented Paper Method: DNA Synthesis and Mutagenesis by Rapid Microscale "Shotgun Gene Synthesis". M.H. Caruthers, A.D. Barone, S.L. Beaucage, D.R. Dodds, E.F. Fisher, L.J. McBride, M. Matteucci, Z. Stabinsky, and J.-Y. Tang, Chemical Synthesis of Deoxyoligonucleotides by the Phosphoramidite Method. S.J. Horvath, J.R. Firca, T. Hunkapiller, M.W. Hunkapiller, and L. Hood, An Automated DNA Synthesizer Employing Deoxynucleoside 3*b7-Phosphoramidites. Site-Specific Mutagenesis and Protein Engineering. M.J. Zoller, Oliconucleotide-Directed Mutagenesis: A Simple Method Using Two Oligonucleotide Primers and a Single-Stranded DNA Template. W. Kramer and H.-J. Fritz, Oligonucleotide-Directed Construction of Mutations via Gapped Duplex DNA. T.A. Kunkel, J.D. Roberts, and R.A. Zakour, Rapid and Efficient Site-Specific Mutagenesis without Phenotypic Selection. P. Carter, Improved Oligonucleotide-Directed Mutagenesis Using M13 Vectors. D.F. Mark, A. Wang, and C. Levenson, Site-Specific Mutagenesis to Modify the Human Tumor Necrosis Factor Gene. R. Pine and P.C. Huang, An Improved Method to Obtain a Large Number of Mutants in a Defined Region of DNA. P. Kollman and W.F. van Gunsteren, Molecular Mechanics and Dynamics in Protein Design. S. Pongor, The Use of Structural Profiles and Parametric Sequence Comparison in the Rational Design of Polypeptides. J.W. Taylor and E.T. Kaiser, Structure*b1Function Analysis of Proteins through the Design, Synthesis, and Study of Peptide Models. C.R. Matthews, Effect of Point Mutations on the Folding of Globular Proteins. T. Alber and B.W. Matthews, Structure and Thermal Stability of Phage T4 Lysozyme. Author Index. Subject Index.