Ternary Complex of Plasmid DNA with Protamine and γ-Polyglutamic Acid for Biocompatible Gene Delivery System

Pharmaceutical Society of Japan

The purpose of the present study was to investigate the usefulness of the ternary complex with protamine and γ-polyglutamic acid (γ-PGA), which are biodegradable materials for foods and medical products, as a safe gene delivery vector. We formed cationic binary complexes (plasmid DNA (pDNA)/protamine complexes) with high transfection efficiency. The binary complex showed slight toxicity probably related to its total cationic charge. Then, we formed ternary complexes (pDNA/protamine/γ-PGA complexes) by addition of anionic polymer, γ-PGA, and they showed no cytotoxicity. The transfection efficiency of the pDNA/protamine/γ-PGA complexes was as high as that of the pDNA/protamine complexes, although their zeta potentials were different. Inhibition study of the gene expressions in B16-F10 cells suggested that pDNA/protamine complexes were taken up by caveolae-mediated endocytosis and macropinocytosis. On the other hand, pDNA/protamine/ γ-PGA complexes were taken up by clathrin-mediated endocytosis and macropinocytosis. Thus, we succeeded in developing the ternary complex as a safe gene delivery vector with biocompatible materials.;開始ページ : 1794;終了ページ : 1799;元資料の権利情報 : © 2013 The Pharmaceutical Society of Japan.

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この本の情報

書名 Ternary Complex of Plasmid DNA with Protamine and γ-Polyglutamic Acid for Biocompatible Gene Delivery System
著作者等 日本薬学会
神田 紘介
書名別名 生体適合型遺伝子デリバリーシステムとしてのプロタミンおよびγ-ポリグルタミン酸を用いたpDNA三重複合体の作製
出版元 Pharmaceutical Society of Japan
刊行年月 1993-
ページ数 v.
大きさ 29 cm
ISSN 09186158
NCID AA10885497
※クリックでCiNii Booksを表示
全国書誌番号
10047547
※クリックで国立国会図書館サーチを表示
言語 英語
出版国 日本
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