Cell culture for biochemists

R. L. P. Adams

This 2nd revised edition equals the popular 1st edition in providing a clear and detailed overview of cell culture. It presents information on: characteristics of cultured cells; culture vessels; glassware preparation and sterilisation techniques; subculturing; primary cells; cell culture media; techniques; contamination; the cell cycle; cell synchronisation; use of radioactive isotopes in cell culture; cell mutants and cell hybrids; viruses; and differentiation in cell cultures. Reviews on the 1st edition: ``.. the book provides an excellent insight into the way cell culture techniques can be employed in the analytical study of cellular biology.'' - Trends in Biochemical Sciences ``It is well written in a concise, easy-to-read style which stimulates the interest of the reader...'' - Science Tools ``A useful handbook on principles and practice.'' - Immunology Today

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  • Acknowledgements. Chapter 1. Introduction. 1.1 Background. 1.2 Some advantages. 1.3 Applications.1.4 Animal cell biotechnology. Chapter 2. Characteristics of cultured cells. 2.1 Types of cells. 2.2 Primary cells and transformation. 2.3 Growth control. 2.4 Attachment and Spreading. 2.5 Growth factors. 2.6 Differentiated functions in cell cultures. Chapter 3. Culture vessels. 3.1 Design of culture vessels. 3.2 Small scale cultures. 3.3 Intermediate scale cultures. 3.4 Large scale cultures. 3.5 Suspension cultures. 3.6 Microcarriers. 3.7 Air lift systems. 3.8 Bioreactors. Chapter 4. Subculturing. 4.1 Dissociation techniques. 4.2 Subculture of a cell monolayer. 4.3 Subculture of cells growing in suspension. 4.4 Protocol for setting up microcarrier cultures 4.5 Subculture of cells growing on microcarriers. 4.6 The growth cycle. Chapter 5. Cell culture media. 5.1 Introduction. 5.2 Balanced salt solutions. 5.3 Eagle's medium. 5.4 More complicated media. 5.5 Simple media with unspecified additives. 5.6 Antibiotics. 5.7 Serum. 5.8 Serum-free media. 5.9 Media for culture of insect cells. 5.10 Media for culture of plant cells. Chapter 6. Primary cells. 6.1 Introduction. 6.2 Lymphocytes. 6.3 Human skin biopsies. 6.4 Mouse or rat embryo cultures. 6.5 Chick embryo cells. 6.6 Chick embryo liver cells. 6.7 Rat hepatocytes. 6.8 Primary kidney cells. 6.9 Endothelial cells. 6.10 Mammary epithelial cell cultures. 6.11 Colonic epithelial cells. 6.12 Rat or chick skeletal muscle cells. 6.13 Mouse macrophage cultures. 6.14 Ascites cells. 6.15 Dipteran cell culture. Chapter 7. Techniques. 7.1 Cell cloning and plating efficiency. 7.2 Cell counting procedures. 7.3 Storage of cells. 7.4 Karyotyping. 7.5 Cell transfection. 7.6 Cell visualisation. 7.7 Sub-cellular fractionation. Chapter 8. Glassware preparation and sterilisation techniques. 8.1 General. 8.2 Sterilisation by heat. 8.3 Sterilisation by filtration. Chapter 9. Contamination. 9.1. Bacterial contamination. 9.2 Sterility checks. 9.3 Analysis of bacterial contamination. 9.4 Airborne contamination. 9.5 Antibiotics. 9.6 Disposal of contaminated material. 9.7 Mycoplasmas. 9.8 Testing for mycoplasma 9.9 Elimination of mycoplasmas. 9.10 Viral contamination. Chapter 10. The cell cycle. 10.1 Description. 10.2 Mitosis. 10.3 S-phase. 10.4 Control of the cell cycle. 10.5 Distribution of cells around the cycle. 10.6 Growth fraction. 10.7 Cell cycle analysis. Chapter 11. Cell synchronisation. 11.1 Intoduction. 11.2 Selection of mitotic cells. 11.3 Selective killing of cells in particular phases. 11.4 Selection of cells by size. 11.5 Synchronisation by subculture. 11.6 Serum deprivation. 11.7 Isoleucine starvation. 11.8 Blockade of S-phase. 11.9 Procedure for inducing synchrony at the G1/S interphase. 11.10 Synchronisation in G2. 11.11 Synchronisation in M. Chapter 12. Use of radioactive isotopes in cell culture. 12.1 Estimation of rates of DNA synthesis. 12.2 Estimation of rates of RNA and protein synthesis.

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書名 Cell culture for biochemists
著作者等 Adams, R. L. P.
Adams R.L.P.
シリーズ名 Laboratory techniques in biochemistry and molecular biology
出版元 Elsevier
刊行年月 1990
版表示 2nd revised ed
ページ数 xvii, 364 p.
大きさ 21 cm
ISBN 0444812970
NCID BA11435880
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言語 英語
出版国 オランダ