Aberrant levels of histone H3 acetylation induce spermatid anomaly in mouse testis

Histone acetylation is involved in the regulation of chromatin structure and gene function. We reported previously that histone H3 acetylation pattern is subject to dynamic changes and limited to certain stages of germ cell differentiation during murine spermatogenesis, suggesting a crucial role for acetylation in the process. In the present study, we investigated the effects of hyper- and hypo-acetylation on spermatogenesis. Changes in acetylation level were induced by either in vivo administration of sodium phenylbutyrate, a histone deacetylase inhibitor, or by knockdown of histone acetyltransferases using short hairpin RNA plasmids transfection. Administration of sodium phenylbutyrate induced accumulation of acetylated histone H3 at lysine 9 and lysine 18 in round spermatids, together with spermatid morphological abnormalities and induction of apoptosis through a Bax-related pathway. Knockdown of steroid receptor coactivator 1, a member of histone acetyltransferases, but not general control of amino acid synthesis 5 nor elongator protein 3 by in vivo electroporation of shRNA plasmids, reduced acetylated histone H3 at lysine 9 in round spermatids, and induced morphological abnormalities. We concluded that the proper regulation of histone H3 acetylation levels is important for spermatid differentiation and complex chromatin remodeling during spermiogenesis.;開始ページ : 209;終了ページ : 224;元資料の権利情報 : (C) Springer-Verlag Berlin Heidelberg 2014;元資料の権利情報 : The final publication is available at link.springer.com



書名 Aberrant levels of histone H3 acetylation induce spermatid anomaly in mouse testis
著作者等 Society for Histochemistry
戴 鐳
書名別名 マウス精巣に於いてヒストン H3 アセチル化レベルの変異が精子細胞異常を引き起こす
出版元 Springer Berlin Heidelberg
刊行年月 c1995-
ページ数 v.
大きさ 28 cm
ISSN 09486143
NCID AA11033302
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言語 英語
出版国 ドイツ

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