Influence of Different Additives and Their Concentrations on Corneal Toxicity and Antimicrobial Effect of Benzalkonium Chloride

Purpose: The aim of this study was to examine the ophthalmic additives responsible for modulating acute corneal epithelial toxicity induced by benzalkonium chloride (BAC) and investigate the ability of polyoxyethylene hydrogenated castor oil 40 (HCO-40) and polysorbate 80 (PS-80) to reduce the corneal toxicity and antimicrobial effects of BAC.Methods: Cytotoxicity of the additives, which included glycerin, polyvinyl alcohol, propylene glycol, polyethylene glycol, and PS-80, on rabbit corneal epithelial cells was examined using the cell proliferation assay in the presence and absence of 0.02% BAC. The corneal transepithelial electrical resistance change after a 60-second exposure to HCO-40 or PS-80 mixed with 0.02% BAC was measured in living rabbits. Corneal damage was examined using scanning electron microscopy. The antimicrobial activities of HCO-40 and PS-80 with 0.02% BAC against Staphylococcus aureus, Propionibacterium acnes, Pseudomonas aeruginosa, Escherichia coli, and Streptococcus pneumoniae were assessed.Results: Of all the tested additives, only PS-80 could prevent the BAC-induced cytotoxicity. Corneal epithelial barrier function disorder caused by 0.02% BAC was significantly alleviated by either PS-80 or HCO-40 in a concentration-dependent manner. Scanning electron microscopy images showed an improvement of BAC-induced corneal epithelial toxicity after the addition of HCO-40 or PS-80. The antimicrobial effect of the BAC against P. aeruginosa, E. coli, and S. pneumoniae was reduced after adding HCO-40 or PS-80.Conclusions: HCO-40 and PS-80 reduce acute corneal toxicity and the antimicrobial effect of BAC. Possible interactions between BAC and other additives should be taken into consideration when evaluating the toxicity and antibacterial properties of BAC.;開始ページ : 521;終了ページ : 526;元資料の権利情報 : © 2014 by Lippincott Williams & Wilkins.;元資料の権利情報 : This is a non-final version of an article published in final form in Cornea, 33(5), pp.521-526; 2014.

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書名 Influence of Different Additives and Their Concentrations on Corneal Toxicity and Antimicrobial Effect of Benzalkonium Chloride
著作者等 鬼塚 尚子
書名別名 塩化ベンザルコニウムに起因する角膜上皮障害と防腐効果に対する異なる2種類の添加剤とその濃度の影響
出版元 Lippincott Williams & Wilkins
刊行年月 c1982-
ページ数 v
ISSN 02773740
NCID AA10623220
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言語 英語
出版国 アメリカ合衆国
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