Hepatocyte growth factor enhances the barrier function in primary cultures of rat brain microvascular endothelial cells

The effects of hepatocyte growth factor (HGF) on barrier functions were investigated by a blood-brain barrier (BBB) in vitro model comprising a primary culture of rat brain capillary endothelial cells (RBEC). In order to examine the response of the peripheral endothelial cells to HGF, human umbilical vascular endothelial cells (HUVEC) and human dermal microvascular endothelial cells (HMVEC) were also treated with HGF. HGF decreased the permeability of RBEC to sodium fluorescein and Evans blue albumin, and dose-dependently increased transendothelial electrical resistance (TEER) in RBEC. HGF altered the immunochemical staining pattern of F-actin bands and made ZO-1 staining more distinct on the linear cell borders in RBEC. In contrast, HGF increased sodium fluorescein and Evans blue albumin permeability in HMVEC and HUVEC, and decreased TEER in HMVEC. In HMVEC, HGF reduced cortical actin bands and increased stress fiber density, and increased the zipper-like appearance of ZO-1 staining. Western blot analysis showed that HGF significantly increased the amount of ZO-1 and VE-cadherin. HGF seems to act on the BBB to strengthen BBB integrity. These findings indicated that cytoskeletal rearrangement and cell-cell adhesion, such as through VE-cadherin and ZO-1, are candidate mechanisms for the influence of HGF on the BBB. The possibility that HGF has therapeutic significance in protecting the BBB from damage needs to be considered.;開始ページ : 41;終了ページ : 49;元資料の権利情報 : © 2013 Elsevier Inc.;元資料の権利情報 : NOTICE: this is the author's version of a work that was accepted for publication in Microvascular Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Microvascular Research, 92, (2014)

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書名 Hepatocyte growth factor enhances the barrier function in primary cultures of rat brain microvascular endothelial cells
著作者等 山田 成美
書名別名 肝細胞増殖因子は初代培養ラット脳毛細血管内皮細胞のバリア機能を強化する
出版元 Academic press Inc.
刊行年月 1968-
ページ数 v.
大きさ 26cm.
ISSN 00262862
NCID AA00738587
※クリックでCiNii Booksを表示
言語 英語
出版国 アメリカ合衆国
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