TGFα, c-MYC, mutated CTNNB1 and their combinations act distinctly on the Hep3B tumors in nude mice

Osaka University Medical School

Hepatocellular carcinoma (HCC) is the most common hepatic tumor worldwide and is a major cause of death in many countries. Although chronic viral infections and hepatotoxic agents are the major risk factors, the molecular pathogenesis of HCC remains largely unknown. Among model mice, TGFα/c-Myc transgenic mice develop human HCC-like tumors. Whole genome sequencing of HCC tumors from patients has shown that mutations to CTNNB1 (β-catenin) are the most frequent (15.9%). So in this study, we characterized the effects of TGFα, c-MYC and mutant CTNNB1 genes on the transplantable Hep3B cells. TGFα increased the tumor number after transplanting Hep3B cells. Mutant CTNNB1 changed the characteristics of Hep3B tumor cells into a disarranged structure with loose cell-cell contacts. And co-transduction of TGFα and mutant CTNNB1 synergistically increased the growth rate, size and weight of Hep3B tumors. Unexpectedly, c-MYC overexpression in the Hep3B cells induced apoptosis. Interestingly, TGFα+mutant CTNNB1+MYC-transduced cells grew very slowly and the tumor showed transdifferentiation into a cholangial duct. These results suggest that the interaction of these three genes determines the characteristics of Hep3B tumor. Overexpression of c-MYC by a lentivirus inc-MYC-upregulated HCC tumors might be used as a molecular targeted therapy.;浜松医科大学学位論文 医博第668号(平成26年3月17日)

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書名 TGFα, c-MYC, mutated CTNNB1 and their combinations act distinctly on the Hep3B tumors in nude mice
著作者等 則武 秀尚
Noritake, Hidenao
書名別名 TGFα,c-MYC,変異型CTNNB1遺伝子およびこれらの組み合わせはヌードマウスにおけるHep3B腫瘍形成に明確に作用する
出版元 大阪大学
刊行年月 1949-
ページ数 v.
大きさ 26 cm
ISSN 00306169
NCID AA00727376
※クリックでCiNii Booksを表示
全国書誌番号
10012411
※クリックで国立国会図書館サーチを表示
言語 英語
出版国 日本
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