RANKL pretreatment plays an important role in the differentiation of pit-forming osteoclasts induced by TNF-α on murine bone marrow macrophages

BackgroundOsteoclasts differentiated from bone marrow macrophages (BMMs) induced by TNF-α alone do not have resorbing activity. When BMMs are stimulated with receptor activator of NF-κB ligand (RANKL) before TNF-α stimulation, pit-forming osteoclasts are differentiated. However, the details of the effect of RANKL pretreatment on the pit-forming osteoclast differentiation by TNF-α have not been established. The aim of this study is to examine the condition of RANKL pretreatment for differentiation of pit-forming osteoclasts induced by TNF-α.Murine BMMs were stimulated with various concentrations of RANKL for 24 h in the presence of M-CSF, then the medium was changed and TNF-α was added. Osteoclasts and pits formation were examined. Osteoprotegerin (OPG), decoy receptor of RANKL, was added to the culture to examine the necessity of co-existing RANKL with TNF-α on the formation of pit-forming osteoclasts. To investigate the influence of RANKL of sufficient concentration as pretreatment for pit-forming osteoclast formation by TNF-α, dose- and time-dependent changes of osteoclast formation were checked.ResultsThe pit formation by osteoclasts in response to TNF-α required 10 ng/mL RANKL pretreatment. Stimulation with this concentration of RANKL led to the differentiation of mature osteoclasts in the 72 h culture. The pit formation was not inhibited by the OPG.ConclusionThese results suggested that the concentration of RANKL pretreatment, which also alone can differentiate BMMs into osteoclasts, may be important in the differentiation of pit-forming osteoclasts by TNF-α. In addition, the effects of TNF-α after RANKL treatment might be independent of RANKL.;開始ページ : 1273;終了ページ : 1282;元資料の権利情報 : © 2015 Elsevier Ltd.

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書名 RANKL pretreatment plays an important role in the differentiation of pit-forming osteoclasts induced by TNF-α on murine bone marrow macrophages
著作者等 Elsevier Science Publishers
山下 恭德
書名別名 RANKL 前刺激はマウス骨髄マクロファージにおいてTNF-α 刺激による吸収活性を持つ破骨細胞の分化に重要な役割を持つ
出版元 Elsevier Ltd.
刊行年月 1959-
ページ数 v
大きさ 24-28 cm
ISSN 00039969
NCID AA11521918
AA00548457
※クリックでCiNii Booksを表示
言語 英語
出版国 イギリス
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