Intratracheal administration of recombinant human keratinocyte growth factor promotes alveolar epithelial cell proliferation during compensatory lung growth in rat

Keratinocyte growth factor (KGF) is considered to be one of the most important mitogens for lung epithelial cells. The objectives of this study were to confirm the effectiveness of intratracheal injection of recombinant human KGF (rhKGF) during compensatory lung growth and to optimize the instillation protocol. Here, trilobectomy in adult rat was performed, followed by intratracheal rhKGF instillation with low (0.4 mg/kg) and high (4 mg/kg) doses at various time-points. The proliferation of alveolar cells was assessed by the immunostaining for proliferating cell nuclear antigen (PCNA) in the residual lung. We also investigated other immunohistochemical parameters such as KGF, KGF receptor and surfactant protein A as well as terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling. Consequently, intratracheal single injection of rhKGF in high dose group significantly increased PCNA labeling index (LI) of alveolar cells in the remaining lung. Surprisingly, there was no difference in PCNA LI between low and high doses of rhKGF with daily injection, and PCNA LI reached a plateau level with 2 days-consecutive administration (about 60%). Our results indicate that even at low dose, daily intratracheal injection is effective to maintain high proliferative states during the early phase of compensatory lung growth.;開始ページ : 179;終了ページ : 185;元資料の権利情報 : © 2013 The Japan Society of Histochemistry and Cytochemistry

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書名 Intratracheal administration of recombinant human keratinocyte growth factor promotes alveolar epithelial cell proliferation during compensatory lung growth in rat
著作者等 日本組織細胞化学会
古川 克郎
書名別名 keratinocyte growth factor蛋白の気管内投与はラットの代償性肺肥大における肺胞上皮細胞増殖を促進する
出版元 日本組織細胞化学会
刊行年月 [1968]-
ページ数 v.
大きさ 29cm
ISSN 00445991
NCID AA00508022
AA11504078
※クリックでCiNii Booksを表示
全国書誌番号
10021621
※クリックで国立国会図書館サーチを表示
言語 英語
出版国 日本
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