Analysis of a Lys-specific serine endopeptidase secreted via the type IX secretion system in Porphyromonas gingivalis

Federation of European Microbiological Societies

Porphyromonas gingivalis, a significant causative agent of adult periodontitis, possesses a novel secretion system called the type IX secretion system (T9SS). A number of virulence factors, such as Arg-gingipain (Rgp), are translocated onto the cell surface and into the extracellular milieu via the T9SS. In this study, we found that the PGN_1416 90- to 120-kDa diffuse protein bands were located in the outer membrane fraction and that the presence of the bands was dependent on genes involved in the T9SS and the formation of anionic lipopolysaccharide (A-LPS). These data strongly suggest that the PGN_1416 protein is secreted by the T9SS and anchored onto the cell surface by binding to A-LPS. Enzymatic analysis using outer membrane fractions suggested that the PGN_1416 protein has a Lys-specific serine endopeptidase activity and that its activation requires processing by Rgp. Homologues of the gene encoding PGN_1416, which is referred to as pepK, were found in bacteria belonging to the phyla Bacteroidetes and Proteobacteria, whereas homologues encoding the C-terminal domain, which is essential for T9SS-mediated secretion, and the catalytic domain were only observed in bacteria belonging to the Bacteroidetes phylum.;開始ページ : 60;終了ページ : 68;元資料の権利情報 : © 2014 Federation of European Microbiological Societies;元資料の権利情報 : This is a pre-copyedited, author-produced PDF of an article accepted for publication in FEMS Microbiology Letters following peer review. The version of record FEMS Microbiology Letters, 354 (2014) 60-68; 2014 is available online at: http://dx.doi.org/10.1111/1574-6968.12426

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この本の情報

書名 Analysis of a Lys-specific serine endopeptidase secreted via the type IX secretion system in Porphyromonas gingivalis
著作者等 Federation of European Microbiological Societies
FEMS Congress of European Microbiologists
野中 美那子
書名別名 ポルフィロモナス・ジンジパリスにおける9型分泌機構依存性分泌ペプチダーゼ、リシン特異的セリンエンドペプチダーゼの解析
出版元 Federation of European Microbiological Societies
刊行年月 1977-
ページ数 v.
大きさ 28 cm
ISSN 03781097
NCID AA11979483
AA00193861
AA11527674
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言語 英語
出版国 オランダ
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