Studies on the establishment of mouse embryonic stem (ES) cell lines and their developmental potency

[目次]

  • CONTENTS
  • I.INTRODUCTION / p1
  • II.ESTABLISHMENT OF EMBRYONIC STEM(ES) CELL LINES / p4
  • INTRODUCTION / p4
  • MATERIALS AND METHODS / p4
  • Culture media / p4
  • Collection of embryos / p5
  • Preparation of feeder layers / p5
  • Isolation and expansion of ES cell lines / p6
  • Alkaline phosphatase staining / p9
  • Production of embryoid body / p9
  • Karyotype analysis of ES cell lines / p9
  • Production of chimeric mice / p10
  • Analysis of the chimerism by GPI analysis / p10
  • Breeding tests on chimeric mice / p11
  • Statistical analysis / p11
  • RESULTS / p12
  • Establishment of ES cell lines / p12
  • In vitro examination / p12
  • In vivo examination / p18
  • DISCUSSION / p24
  • III.PRODUCTION OF MICE DERIVED ENTIRELY FROM ES CELLS AFTER INJECTING THE CELLS INTO HEAT TREATED BLASTOCYSTS / p26
  • INTRODUCTION / p26
  • MATERIALS AND METHODS / p27
  • Production of heat-treated blastocysts / p27
  • Injection of ICM and ES cells into heat treated blastocysts / p28
  • Statistical analysis / p29
  • RESULTS / p29
  • Injection of ICM and ES cells into heat treated blastocysts / p29
  • DISCUSSION / p34
  • IV.PRODUCTION OF MICE DERIVED ENTIRELY FROM ES CELLS AFTER INJECTING THE CELLS INTO TETRAPLOID BLASTOCYSTS / p36
  • INTRODUCTION / p36
  • MATERIALS AND METHODS / p37
  • Production of tetraploid blastocysts / p37
  • Injection of ES cells into tetraploid blastocysts / p37
  • RESULTS / p39
  • DISCUSSION / p39
  • V.NUCLEAR TRANSFER OF ES CELLS INTO ENUCLEATED OOCYTES / p44
  • INTRODUCTION / p44
  • MATERIALS AND METHODS / p45
  • Donor cells / p45
  • Nuclear transfer / p45
  • Serial nuclear transfer into enucleated 2-cell embryos / p48
  • Embryo transfer,GPI analysis, and DNA genotyping / p48
  • Statistical analysis / p48
  • RESULTS / p50
  • In vitro developmental ability of nuclear transferred oocytes received NARA 5 ES cell nuclei / p50
  • In vivo developmental ability of nuclear transferred oocytes received NARA 5 ES cell nuclei / p54
  • In vitro and in vivo developmental ability of enucleated oocytes receiving ES cells from different cell lines / p57
  • DISCUSSION / p65
  • VI.GENERAL DISCUSSION / p70
  • REFERENCE / p74
  • SUMMARY / p83
  • ACKNOWLEDGEMENT

「国立国会図書館デジタルコレクション」より

この本の情報

書名 Studies on the establishment of mouse embryonic stem (ES) cell lines and their developmental potency
著作者等 天野 朋和
書名別名 マウス胚性幹細胞株の樹立と個体発生に関する研究
刊行年月 2000
言語 英語
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