Identification and characterization of multiple HLA-A24-restricted HIV-1-specific CTL epitopes

[目次]

  • Abstract
  • Contents
  • Introduction / p1
  • I. Materials and methods / p6
  • I-1 Cells / p6
  • I-2 Peptides / p7
  • I-3 Peptide binding assay / p8
  • I-4 Evaluation of HLA-A﹡2402 binding peptides / p10
  • I-5 Preparation of PBL / p10
  • I-6 Induction of peptide-specific CTL / p12
  • I-7 CTL assay / p12
  • I-8 Generation of CTL clones / p14
  • II Identification of HIV-1 specific,HLA-A24-restricted CTL epitopes / p15
  • 11-1 Reverse immunogenetics / p15
  • II-2 Binding of synthetic peptides to HLA-A﹡2402 molecule / p18
  • II-3 Induction of peptide-specific CTL from PBLs of HIV-1 seropositive individuals / p21
  • II-4 Generation and characterization of peptide specific CTL clones / p25
  • III Investigation for immunodominant epitopes / p38
  • IV Effects of mutations of immunodominant epitopes on recognition by the specific CTL clones / p42
  • IV-1 Variation of amino acid sequences in HIV-1 clade B strains / p42
  • IV-2. Binding affinity and CTL-recognition of the variant peptides / p46
  • SF2-Env385-9 / p46
  • SF2-Env584-8,-9,and-11 / p47
  • SF2-Env679-9 / p47
  • SF2-Env766-9 / p48
  • SF2-Nef138-10 / p49
  • V Discussion / p53
  • References / p57
  • Acknowledgment / p64

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この本の情報

書名 Identification and characterization of multiple HLA-A24-restricted HIV-1-specific CTL epitopes
著作者等 モア 由紀
書名別名 HLA-A24拘束性・HIV-1特異的CTLエピトープの同定と特性の解析
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