Studies on the relationship between structure and functions of glutathione S-transferase

[目次]

  • CONTENTS / p3
  • Acknowedgments / p2
  • Contents / p3
  • Abbreviations / p6
  • 1.INTRODUCTION / p7
  • 1.1.Introduction to glutathione S-transferase / p8
  • 1.2.Catalytic mechanism of glutathione S-transferase / p11
  • 1.3.Brief description of the present study / p13
  • Table and figures / p15
  • 2.MATERIALS AND METHODS / p18
  • 2.1.Materials / p19
  • 2.2.Synthesis of oligonucleotides / p20
  • 2.3.Polymerase chain reaction / p20
  • 2.4.Construction of expression plasmid pKK-GSTπ and transformation / p21
  • 2.5.Construction of mutant plasmids / p21
  • 2.6.DNA sequence analysis / p21
  • 2.7.Purification of enzyme / p22
  • 2.8.Enzyme assays / p22
  • 2.9.Heat inactivation assays / p24
  • 2.10.Reaction of N-acetylimidazole with hGST P1-1 / p25
  • 2.11.Electrophoresis and blotting / p25
  • 2.12.Amino acid sequence analysis / p26
  • 2.13.Circular dichroism analysis / p26
  • Table and Figure / p27
  • 3.RESULTS AND DISCUSSION / p30
  • 3.1.Expression of recombinant hGST P1-1 in E.coli / p31
  • 3.2.Effects of substitutions of histidine residues / p38
  • 3.3.Effects of substitutions of cysteine residues / p46
  • 3.4.Effects of chemical modification and substitution of tyrosine residues / p53
  • 3.5.Effects of substitutions of evolutionally conserved aspartic acids / p65
  • 3.6.Effects of substitutions of arginine residues / p82
  • 3.7.Effects of substitutions of amino acid residues involved in the GSH binding / p97
  • 4.TOTAL DISCUSSION / p107
  • 5.CONCLUSIONS / p126
  • 6.REFERENCES / p128

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この本の情報

書名 Studies on the relationship between structure and functions of glutathione S-transferase
著作者等 孔 光勲
書名別名 グルタチオンS-トランスフェラーゼの構造・機能相関の研究
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