Development of membrane excitability during the in vitro differentiation of a clonal mouse myogenic cell line

[目次]

  • CONTENTS / p1
  • I.INTRODUCTION / p2
  • II.MATERIALS AND METHODS / p7
  • 1.Cell culture media and culture conditions / p8
  • 2.Establishment of cell lines and selection of a clone / p8
  • 3.Cell culture for experiments / p10
  • 4.Assessments of cell growth, mitotic index, and fusion index / p11
  • 5.Glycogen phosphorylase staining / p11
  • 6.Intracellular recording / p12
  • III.RESULTS / p15
  • 1.Establishment of a clonal myogenic cell line, MC3T3-A1/M13 / p16
  • 2.Growth characteristics of MC3T3-A1/M13 cells / p17
  • 3.Myotube formation of MC3T3-A1/M13 cells / p18
  • 4.Glycogen phosphorylase enzymatic histochemistry / p20
  • 5.Resting membrane potential, specific membrane resistance, and specific membrane capacitance / p20
  • 6.Development of delayed rectification system in MC3T3-A1/M13 myotubes / p22
  • 7.Development of action potential mechanism in MC3T3-A1/M13 myotubes / p22
  • 8.Effects of tetrodotoxin and tetraethylammonium on the action potential and delayed rectification / p24
  • 9.Membrane electrical properties of MC3T3-A1/M13 myotubes under an elevated external Ca concentration condition / p25
  • 10.Effects of external Ca concentration on the action potential and the afterhyperpolarization / p26
  • 11.Effects of Ca, Na, and K channel blockers on the action potential and the afterhyperpolarization / p28
  • 12.Voltage-clamp recordings of Ca current in MC3T3-A1/M13 myotubes / p29
  • 13.Time course of Ca, Na, and Ca-activated K channel development in MC3T3-A1/M13 myotubes / p31
  • IV.DISCUSSION / p34
  • 1.The established myogenic cell lines / p35
  • 2.Resting membrane potential and development of excitability / p36
  • 3.Tetrodotoxin-insensitive Na channel in skeletal muscle cell / p38
  • 4.Voltage-sensitive Ca channel in skeletal muscle cell / p40
  • 5.Time course of Na and Ca channel development / p42
  • 6.Role of Ca-activated K channel / p44
  • V.SUMMARY / p47
  • VI.ACKNOWLEDGMENTS / p51
  • VII.REFERENCES / p53
  • VIII.TABLE,FIGURES AND FIGURE LEGENDS

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この本の情報

書名 Development of membrane excitability during the in vitro differentiation of a clonal mouse myogenic cell line
著作者等 天貝 裕地
書名別名 マウスクローン筋原性細胞株の培養下での分化に伴う興奮性発達
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