Experimental simulation of cleavage in sea urchin egg by calyculin A

[目次]

  • CONTENTS / p3
  • INTRODUCTION / p5
  • MATERIALS AND METHODS / p11
  • Collection of sea urchin gametes / p11
  • Calyculin A / p12
  • Fluorescence microscopy / p12
  • Transmission electron microscopy / p15
  • Scanning electron microscopy / p15
  • Isolation of contractile ring-like structure / p16
  • Immunoblot analysis / p17
  • Assay of actin polymerization / p18
  • Measurements of intracellular calcium and pH / p20
  • Assay of the histone Hi kinase activity / p21
  • Labeling of cyclin / p22
  • Chemicals / p22
  • RESULTS / p24
  • Cleavage-like morphological change / p24
  • Microfilamentous arrangement in the constriction / p25
  • Localization of myosin and other proteins / p27
  • Microtubular structures / p28
  • Effect of calyculin A on actin polymerization / p28
  • Surface movement of microvilli / p29
  • Intracellular calcium and pH / p30
  • Sperm receptors in the constriction / p31
  • Chromosome condensation / p32
  • Histone Hi kinase activities / p33
  • Cyclin synthesis / p33
  • DISCUSSION / p34
  • Cytokinesis / p34
  • Mitosis / p39
  • SUMMARY / p43
  • REFERENCES / p45
  • TABLE AND FIGURES / p61
  • TABLE / p61
  • LEGENDS TO FIGURES / p62
  • FIGURES (1-19) / p69

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この本の情報

書名 Experimental simulation of cleavage in sea urchin egg by calyculin A
著作者等 搭筋 弘章
書名別名 カリクリンAによってウニ卵にひきおこされる卵割類似現象の研究
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