Studies on maturation of bovine oocytes in vitro in the presence of growth hormone

[目次]

  • PREFACE / p1
  • ACKNOWLEDGMENTS / p2
  • CONTENTS / p3
  • LIST OF TABLES / p6
  • LIST OF FIGURES / p8
  • ABSTRACT / p9
  • INTRODUCTION / p1
  • CHAPTER 1. GENERAL METHODOLOGY / p4
  • Media / p4
  • Collection and maturation of oocytes / p4
  • Preparation of oocytes and spermatozoa for in vitro fertilization / p5
  • Culture of embryos / p5
  • Evaluation of cumulus expansion / p6
  • Assessment for oocyte nuclear maturation and sperm penetration / p6
  • Evaluation of embryo development / p6
  • Count of cell number of blastocysts / p7
  • Statistical analysis / p7
  • CHAPTER 2.EFFECTS OF RECOMBINANT BOVINE GROWTH HORMONE ON NUCLEAR MATURATION OF BOVINE OOCYTES IN VITRO AND SUBSEQUENT EMBRYONIC DEVELOPMENT / p8
  • Materials and Methods / p9
  • Results / p10
  • Discussion / p15
  • Summary / p19
  • CHAPTER 3.EFFECTS OF RECOMBINANT HUMAN INSULIN-LIKE GROWTH FACTOR-I ON NUCLEAR MATURATION OF BOVINE OOCYTES IN VITRO AND SUBSEQUENT EMBRYONIC DEVELOPMENT / p21
  • Materials and Methods / p22
  • Results / p23
  • Discussion / p26
  • Summary / p31
  • CHAPTER 4.POSSIBLE INVOLVEMENT OF INSULIN-LIKE GROWTH FACTOR-I IN MEDIATING THE STIMULATORY EFFECT OF RECOMBINANTBOVINE GROWTH HORMONE ON MATURATION OF BOVINE OOCYTES IN VITRO / p33
  • Materials and Methods / p34
  • Results / p35
  • Discussion / p40
  • Summary / p42
  • CONCLUSION / p43
  • REFERENCES / p44
  • ABSTRACT IN JAPANESE / p53

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この本の情報

書名 Studies on maturation of bovine oocytes in vitro in the presence of growth hormone
著作者等 伊賀 浩輔
書名別名 ウシ卵母細胞の成長ホルモン存在下での体外成熟に関する研究
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