Biotransformation of benzene into cis-benzene glycol by a genetically modified Pseudomonas

[目次]

  • 論文の内容の要旨
  • Table of Contents / p2
  • Introduction. / p1
  • Part1.Characterization of a cis-benzene glycol producing strain. / p3
  • Chapter1.Biological oxidation of aromatic hydrocarbons. / p3
  • 1.1.Metabolism of aromatic hydrocarbons in eukaryotes. / p3
  • 1.2.Metabolism of hydrocarbons in prokaryotes. / p10
  • 1.3.Anaerobic degradation of aromatic compounds. / p25
  • 1.4.Conclusions. / p28
  • Chapter2.Strain isolation. / p29
  • 2.1.Aromatics and benzene degraders. / p29
  • 2.2.Growth of bacteria with benzene as carbon source: apparatus and media. / p29
  • 2.3.Isolation of benzene degraders. / p33
  • 2.4.Strain choice and preliminary identification. / p35
  • 2.5.Utilization of various carbon sources by the strain JI104. / p40
  • 2.6.Characterization of the strain Pseudomonas JI104. / p40
  • 2.7.Conclusions. / p46
  • Chapter3.Localization of the benzene genes. / p47
  • 3.1.Benzene degraders. / p47
  • 3.2.Stability of the benzene degradation pathway of Pseudomonas JI104. / p48
  • 3.3.Plasmid isolation. / p49
  • 3.4.Conjugation to other strains. / p52
  • 3.5.Cloning attempts. / p54
  • 3.6.Conclusions. / p55
  • Chapter4.Gene tagging and transposon mutagenesis. / p56
  • 4.1.Transposon mutagenesis:interest. / p56
  • 4.2.Targets of the transposon mutation. / p57
  • 4.3.Screening methods. / p58
  • 4.4.Transposition mutagenesis systems. / p60
  • 4.5.Optimization of the system. / p66
  • 4.6.Mutants isolation. / p71
  • 4.7.Mutants characterization. / p72
  • 4.8.Effect of benzoate as inducer. / p77
  • 4.9.Conclusions. / p78
  • Chapter5.Chemical mutagenesis. / p79
  • 5.1.Mutation method. / p79
  • 5.2.Mutants isolation and characterization. / p81
  • 5.3.Identification of cis-benzene glycol. / p86
  • 5.4.cis-Benzene glycol producing mutants. / p87
  • 5.5.Conclusion. / p89
  • Chapter6.Hydrocarbon utilisation pathways and their regulation. / p94
  • 6.1.Detection of cis-benzene glycol as the first benzene metabolite. / p94
  • 6.2.Induction of the different pathways. / p96
  • 6.3.A constitutive meta pathway. / p98
  • 6.4.Effect of benzoate on mutant strains. / p99
  • 6.5.Indole oxidation. / p107
  • 6.6.Phenol generation. / p109
  • 6.7.Conclusions. / p110
  • Part2.Towards a cis-benzene glycol production process. / p114
  • Chapter7.Factors affecting cis-benzene glycol production. / p114
  • 7.1.Choice of carbon source for a production process. / p114
  • 7.2.Effect of carbon sources concentration. / p116
  • 7.3.Growth rates of the mutant strain with different media and carbon sources. / p120
  • 7.4.Effect of iron on the cis-benzene glycol production. / p123
  • 7.5.Effect of Nitrogen source on cBG production. / p126
  • 7.7.Optimum pH. / p126
  • 7.7.Effect of temperature on growth and production. / p129
  • 7.8.Conclusions. / p132
  • Chapter8.Effect of benzene concentration. / p133
  • 8.1.Effect on the growth of the wild type. / p133
  • 8.2.Benzene concentration influence on indole oxidation. / p134
  • 8.3.Apparatus and growth methods. / p136
  • 8.4.Influence of benzene concentration on the initial productivity of the strain JI104-16. / p138
  • 8.5.Evolution of the productivity during the fermentation. / p140
  • 8.6.Oxygen demand during exposure to benzene. / p143
  • 8.8.Conclusions. / p148
  • Chapter9.Benzene toxicity. / p149
  • 9.1.Observed effects and possible mechanisms. / p149
  • 9.2.Benzene interaction with the membrane. / p151
  • 9.3.Adsorption to the cells macromolecules,DNA,RNA,and proteins. / p152
  • 9.4.Estimation of the stability of the benzene dioxygenase system. / p153
  • 9.5.Inactivation of the reaction site by benzene metabolites. / p158
  • 9.6.Inactivation of the active site by oxygen radicals. / p163
  • 9.7.cis-Benzene glycol production under a reduced oxygen concentration. / p166
  • 9.8.Conclusions. / p169
  • Chapter10.Perspectives towards a production process. / p170
  • 10.1.Outline of a process. / p170
  • 10.2.Benzene transformation to cis-benzene glycol. / p171
  • 10.3.Product recovery. / p173
  • 10.4.Perspectives for further studies. / p174
  • Summary and concluding remarks. / p176
  • Bibliography. / p181
  • Annexes. / p208

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この本の情報

書名 Biotransformation of benzene into cis-benzene glycol by a genetically modified Pseudomonas
著作者等 Jean-Jacques
Yarmoff
Yarmoff, Jean-Jacques
書名別名 Pseudomonasによるシス-ベンゼンのベンゼングリコールへの転換
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